FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16910503
Sequences flagged as poor quality0
Sequence length42
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10918556.456667788060473No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC1009360.5968834871440548No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532370.31481618258191374No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329940.19510951270934992No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311540.18422870094402277No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300940.17796040720964953No Hit
TCTTTTAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC239990.14191771823700336No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183980.10879629068396132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC501250.027.5169936
ACGACGC9100.022.54888510
CGCACGA1300.022.1536857
CGACGCT9600.021.93695311
CACGACG9600.021.3744669
TCATAAC650100.021.31070535
GCACGAC5700.021.157558
CGAGTTG18050.020.7422317
GACGCTC10250.020.72143412
GCGAGTT11650.020.5520636
GCGACAA19050.020.50318136
TGAGTTG115600.020.4756287
GCCGGGG29400.019.46866836
TGCGAGT12800.018.4242465
TCGCGAA9300.018.19298620
ATCGCGA9450.018.09467719
ACGCTCT11750.017.92290313
CGCGAAG9700.017.62821621
GCGGACT49850.017.58410636
TTGCGAG11800.017.544634
Produced by FastQC (version 0.11.5)