FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16910503
Sequences flagged as poor quality0
Sequence length42
%GC19

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3270781.9341707340106915No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1268110.7498949025939677No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA591010.3494928565992389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377140.22302116028127608No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357770.21156674050440724No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA293080.17331240827076522No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235970.13954049740566557No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224710.13288191368405777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGT147500.034.796519
CAACGAC116800.034.79131310
AAACTGC121050.034.7740410
TACACGG89800.034.71053310
CCACTTT288600.034.66082813
ACGACTT222950.034.6293812
CAGATAG170450.034.5774089
TCCGTTT265600.034.57236513
GAAACTG121150.034.566169
CACTATC134450.034.559999
CGACTTT226000.034.51415313
ACCAGAC108600.034.50171310
CTTCACT196050.034.4964611
ACGCGTA118350.034.48689
GTCCACT149250.034.4854811
GCCTATT144950.034.47883212
TCCACTT155050.034.4730412
GCTTCAC190150.034.42204710
CGCGTAA120750.034.41331510
CAGACTT130850.034.3843812
Produced by FastQC (version 0.11.5)