FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26518849
Sequences flagged as poor quality0
Sequence length16
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAA15238355.7462335563658895No Hit
CAAAAAAAAAAAAAAA6882562.595346426988592No Hit
GGGGGGGGGGGGGGGG5462972.0600328468252904No Hit
CCAAAAAAAAAAAAAA3399971.282095614330773No Hit
TAAAAAAAAAAAAAAA1474090.5558650000231911No Hit
ACAAAAAAAAAAAAAA1085430.4093050946517325No Hit
CTAAAAAAAAAAAAAA1084130.4088148772972764No Hit
TCAAAAAAAAAAAAAA1078910.4068464660739989No Hit
TCCAAAAAAAAAAAAA773210.2915699697222907No Hit
CCCAAAAAAAAAAAAA708570.2671948544976443No Hit
ACCAAAAAAAAAAAAA700940.2643176557172598No Hit
GCAAAAAAAAAAAAAA632360.23845680481833884No Hit
GAAAAAAAAAAAAAAA613880.2314881765796095No Hit
ATAAAAAAAAAAAAAA493830.18621848934695467No Hit
CTCAAAAAAAAAAAAA486130.18331489424748412No Hit
AACAAAAAAAAAAAAA343100.12937967254913665No Hit
GCCAAAAAAAAAAAAA339580.12805231478937867No Hit
ATCAAAAAAAAAAAAA320030.1206801999589047No Hit
ACTAAAAAAAAAAAAA302850.11420178907463141No Hit
CACAAAAAAAAAAAAA298270.1124747156258554No Hit
GTAAAAAAAAAAAAAA290600.1095824332345646No Hit
TACAAAAAAAAAAAAA284570.10730857889043374No Hit
TTAAAAAAAAAAAAAA277850.10477453225816852No Hit
TTCAAAAAAAAAAAAA270740.10209342041956647No Hit
CCTAAAAAAAAAAAAA268570.1012751345278975No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGACA814350.09.681989
CACTCAC151500.09.6805089
ACTCACT149450.09.61610
AACACGC100750.09.5589459
AGACGCT141950.09.55692210
ACCTGTC117350.09.54909910
CTACCGT111650.09.5348899
CTGCGAA138800.09.517929
TAGGGAC231550.09.5148098
CAACCAT104150.09.50134859
CTCGTAC82100.09.5012379
GACTACT90200.09.49630110
ACACGCA102400.09.49292310
ACCCATC61600.09.4892649
TGGCACA164850.09.488049
GGCACAA179300.09.47368410
CTGCTAT88400.09.45774610
AGCTGCA136900.09.45288710
CTGTAAC95350.09.45012910
CAGGGAC193300.09.4471168
Produced by FastQC (version 0.11.5)