FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31001600
Sequences flagged as poor quality0
Sequence length42
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT585887718.89862781275805No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2761900.8908895024772915No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1158580.37371619529314615No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040840.3357375103220479No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC978400.3155966143682907No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870720.28086292320396367No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534750.17249109723369116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422360.13623812964492157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAC28850.028.4469578
CGCACGA5800.027.6171467
CACGACG56050.026.6514389
CATAACC621650.025.61663236
GACGCTC57650.025.56851812
CGACGCT59850.024.6887211
ACGACGC60550.024.64105210
CCGATCT52400.024.59341621
ACGCTCT62150.023.57242613
TCCGATC56000.023.39771520
TTCCGAT66500.022.27433619
CCCACGA13200.021.9517297
CGAGTTG26550.021.6243447
CGCTCTT69450.021.43151314
GCGAGTT19500.020.6744386
CGATCTT59550.020.25072722
TCATAAC805950.019.84120835
GGCACGA27950.019.6396987
TGAGTTG159900.019.6297517
TCGCGAA19600.019.00834820
Produced by FastQC (version 0.11.5)