FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31001600
Sequences flagged as poor quality0
Sequence length42
%GC29

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13523914.362326460569777No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4523291.4590505006193228No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1744630.5627548255573906No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1697880.5476749587118085No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1122950.362223240090834No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA813040.2622574318744839No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA786500.253696583402147No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA755910.24382935074318746No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA487530.15725962530966142No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA444410.1433506657720892No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431330.13913152869529313No Hit
TCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400620.12922558835672998No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA396820.1279998451692816No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA368650.11891321738232866No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA356570.11501664430222958No Hit
CCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330780.10669771882741537No Hit
ACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320760.10346562758051198No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA314620.10148508464079273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG398800.035.294739
TACACGG395800.035.13955310
CAGTGAC535600.035.0220039
AGCTACC331500.034.96650310
CACCGTG262700.034.96211210
AATCGGC395350.034.937949
CACGTCA259400.034.8933510
GCACGTC241700.034.8713389
TCTAGAG390800.034.838019
CGAGAAT210250.034.8132979
AAACTGC201000.034.81254610
TGACATT465200.034.8001812
CAAACCT248350.034.793211
AGCAACT235600.034.77353711
GTGACAT489750.034.77234611
GCTACCT299550.034.7416911
TCGGCAT362600.034.7377311
CGCTATA218650.034.7355239
CGTATCA305100.034.7289669
TCTCGTT436500.034.72517812
Produced by FastQC (version 0.11.5)