FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16550896
Sequences flagged as poor quality0
Sequence length63
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15173899.168017248129647No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT752610.454724626388807No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521560.315124933417502No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485830.29353697830014763No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456140.2755983724385677No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275860.16667375591025405No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190230.11493637564999502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG33700.039.24003257
CACGACG9950.033.5074279
ACGACGC10300.032.36882410
CGGTACG2200.031.086337
CGACGCT10700.030.62818311
GCCGCTA43150.030.5136856
GACGCGA70050.026.73053644
CGAGTGC30950.026.51997657
GCACGAC7650.026.4469288
CGCGATG71050.026.43669946
ACGCGAT71000.026.33257345
GCGCCGC49700.026.20578454
TGACGCG71100.026.17512543
ATCGCGA72800.026.14898719
CATCGCG72800.026.10905518
TCGCGAA73050.026.05989320
GCGATGT72650.025.7755447
GTTGACG71750.025.73978241
CGCGAAG74850.025.43319921
GCATCGC75000.025.34471917
Produced by FastQC (version 0.11.5)