FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26337400
Sequences flagged as poor quality0
Sequence length42
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20912457.940210499138107No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC781230.29662381252515435No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT694220.26358714223879354No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445840.16928018711034498No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398690.15137788847798186No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395500.150166683119822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC482250.027.5748536
CGACGCT9350.025.21872511
ACGACGC9800.024.42801510
CACGACG9950.023.8788539
GCACGAC6950.021.7546528
TCATAAC643300.020.75527435
GACGCTC11450.020.4363712
CGGTACG1151.9645086E-1020.3470927
GCCGGGG50900.020.1565236
TCGCGAA14900.019.69022820
ATCGCGA15100.019.42946619
AGGGGGG52050.018.8226851
GCGACAA18750.018.81539236
GCGAGTT15450.018.7598826
CCGATCT10150.018.44229921
CGCGGCG16200.018.11032131
CGCGAAG16350.017.94393221
TCGCCCC11750.017.92282736
TGAGTTG146050.017.8945967
CCGCGGC16600.017.45693830
Produced by FastQC (version 0.11.5)