FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74093673
Sequences flagged as poor quality0
Sequence length42
%GC17

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41264415.569221814661557No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13099871.7680146589574526No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5398310.7285790785402149No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4824980.6512000019218915No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3581310.4833489628729838No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2525180.3408091268467687No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2394210.3231328537323288No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2253390.3041271823573924No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1403530.18942643051316946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1374730.18553945894948412No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1357660.1832356185122581No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1295540.1748516367922535No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1253420.16916694088036371No Hit
TCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1132970.1529104921009922No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1101590.14867531266806006No Hit
ACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA974870.1315726377878446No Hit
CCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA938640.1266828815464446No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA935200.12621860438744884No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA878260.11853373769174595No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870190.11744457586817163No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801820.10821706733313112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGA1192250.035.256710
CTTACGA1490900.035.250149
CGACTTT1702000.035.2294413
CCTAGAT1193650.035.18945311
CCTCTCA998100.035.1540769
ACTGCTG1135250.035.13886310
AACCATG683200.035.12931410
ACGGTGA1253350.035.11599310
TACTGCT1161100.035.085789
TACGGTG1256000.035.0554289
CTAGATT1215700.035.04646312
CTGTACA1388500.035.0398649
AACAGGT742200.035.0328511
GTACAAT1385550.035.02279311
GACTTTT3009350.035.01097514
TTACGAC1493000.034.97957210
TAGCTCT1401550.034.97480411
AGCAACT828550.034.95046611
TAGGACG648900.034.92555610
ACATGTG523500.034.9061859
Produced by FastQC (version 0.11.5)