FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39253780
Sequences flagged as poor quality0
Sequence length42
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT784079419.97462155237024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6319021.6097863696184163No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3802010.9685716891468796No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC2766820.704854411473239No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2132260.5431986422708845No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2128070.5421312291453205No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1978100.5039259913312807No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1262400.32159960136323174No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT722210.18398482897698004No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT714060.18190859580911697No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637110.16230538816898654No Hit
TCTTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAAC629710.16042021940307405No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT627070.15974767270820797No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT576540.14687502706745695No Hit
TCTTTTAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC550940.1403533621475435No Hit
TTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAA522860.13319991093851344No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG511280.13024987657239634No Hit
TCTTTGTGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC477900.12174623692291545No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436850.11128864532281987No Hit
TTTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC433260.11037408371881638No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429190.10933724089756452No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398280.10146284001184089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC1163900.028.79479236
GCGAGTT33000.026.2910426
TGCGAGT33900.025.4868225
CGAGTTG41600.024.5757967
TTGCGAG35450.023.6108114
TGAGTTG208800.023.4730637
TCGCCCC37650.023.43136836
GCCGGGG80200.022.58344336
CTTTGCG31650.022.237082
TCATAAC1530300.022.1075935
GTGAGTT133050.021.9978586
CGGGTGT74950.020.73030736
CGGCGGG76400.019.98017733
TGTGAGT149300.019.8326285
ATCGCGA78900.019.66437319
TTAGAGT171700.019.614525
CGCGGCG77150.019.57547631
TCGCGAA79300.019.54280920
CATCGCG79750.019.45470818
CCGCGGC77450.019.45287130
Produced by FastQC (version 0.11.5)