FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39253780
Sequences flagged as poor quality0
Sequence length42
%GC18

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23122025.890393230919417No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8001062.0382903251610416No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3342020.8513880701425441No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2795210.7120868359684086No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690940.4307712531124391No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1561270.39773749177786194No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1355850.3454062258462752No Hit
CTCCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1328510.3384412915138364No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1214180.30931543408048856No Hit
ACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA858010.21858022335683341No Hit
TCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA741430.18888117271763383No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA714530.18202832950100603No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA663240.16896207193294505No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640760.16323523492514605No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA617500.15730969093931846No Hit
CCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA583240.14858186905821552No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA562040.1431811152964122No Hit
ACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529060.13477937666130496No Hit
CTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467630.11912992837887204No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA424550.10815518913083019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT563400.035.27129
TGCGTGA670250.035.1939629
TACGCTG537850.035.18617210
GACTTGA450500.035.092649
ACGCTGT534050.035.0257911
CTTGAGT454600.035.01393511
ACGATAG297100.035.0120939
TCCGAAC253150.034.9401869
ACCTGTC370350.034.93501310
ACTTGAG451500.034.9349610
GCGTGAT669500.034.9347410
GCAATCG323450.034.9311569
CAATCGA329300.034.9061910
TCAGCGT458450.034.8136810
GACTACT364050.034.7732110
AATCGAT332500.034.73850611
CGATAGG296250.034.73562210
CCGAACA257850.034.7289110
GCAAACT353550.034.7270611
CGCTGTT532650.034.72576512
Produced by FastQC (version 0.11.5)