FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452849
Sequences flagged as poor quality0
Sequence length42
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247890.5566997668234427No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACC110760.24873962714657516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACC63150.026.87873536
GCGACAA8250.022.69080236
TCGCGAA2100.021.4282320
TCATAAC81900.020.79111135
ATCGCGA2300.019.56490719
CGCGAAG2350.019.14863221
CATCGCG2350.019.14863218
GCGAGTT2000.018.8996986
ACGGCCG6500.018.8306833
CGAGTTG2900.018.6203947
ACCGACA500.00824279917.99991612
TTCGGCG802.0490637E-517.9997147
TCGTACG1553.092282E-1117.41907718
GACGGCC6950.017.35243632
GCGAAGG2700.017.33305722
CGAAGGC2600.017.30741723
CGCGGCG2400.017.2499231
CGCGAGG4700.017.23396136
GCCGGGG7250.017.13095536
GCATCGC2650.016.98086417
Produced by FastQC (version 0.11.5)