FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29990645
Sequences flagged as poor quality0
Sequence length63
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334143811.141600989241812No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1382370.4609337345028758No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAA1201550.40064160007228916No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890600.2969592684652164No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT707410.23587688760945288No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT685150.22845457308437347No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT612730.20430704307960032No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT501510.16722214543901942No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440430.1468557945319282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG437280.14580546700479433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT432460.1441982991696244No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT342140.1140822413122492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG21600.035.8881557
ACGCCGT7500.031.15935957
GCACGAC14700.030.2442768
CACGACG31750.030.0702489
CGACGCT32000.028.76659611
ACGACGC33450.028.54201310
TGAGTTG232200.028.3767247
GACGCTC35350.026.52420812
CCGATCT29500.025.6985921
CGAGTTG43450.024.8591387
TCCGATC32200.024.34033220
CTTTCGC159000.023.21178857
GCGCCGC33600.023.15577554
GCTCATC1467750.022.33631923
TATGCTC1489600.022.19795220
ATGCTCA1495500.021.99984421
ATCCCTA1483550.021.93872827
CTCATCC1498900.021.8549824
GAGTTGT1399400.021.7991818
TCATCCC1505450.021.66330325
Produced by FastQC (version 0.11.5)