FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41371228
Sequences flagged as poor quality0
Sequence length42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA1123100.27146885753548333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG772840.18680615426740535No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG579760.14013603850482756No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG499320.12069257407587707No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC490030.1184470521397141No Hit
GATCAATCGCCCCCGGGGGTGCCTCCGGGCTCCTCGGGGTGC434100.10492799488572106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT61150.022.3903241
TCAATCG78550.020.5712223
GGCGATC73950.018.2726822
CAATCGC90600.017.7369084
GATCAAT103500.016.7922521
ATCAATC107750.015.7969922
AATCGCC116600.014.1841275
CGATCAA30400.013.7305071
CGGTGGC341550.013.5018992
TGGCGCG339850.012.8731055
CGATCTG114100.012.2070284
GGCGCGT393750.011.2484856
GTGGCGC397100.011.1840724
GGTGGCG426600.010.8529783
GCGATCT135100.010.5753593
GCGTGCC443100.09.8428389
AGGGCGA98600.09.5268264
CGTGCCT468750.09.42352710
CGCGTGC473350.09.373198
TCGCAGG134250.09.29229135
Produced by FastQC (version 0.11.5)