FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38102712
Sequences flagged as poor quality0
Sequence length42
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN661190.17352833047684374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444190.11657700375763279No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG422340.11084250380917768No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA422340.11084250380917768No Hit
GATCAATCGCCCCCGGGGGTGCCTCCGGGCTCCTCGGGGTGC382550.10039967758725415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG66700.021.6380833
CAATCGC77000.018.7219434
GATCAAT80500.018.6833761
ATCAATC86650.017.5260282
GGGCGAT43150.014.8010511
AATCGCC104000.014.3474395
GGCGATC45100.013.9637182
CGATCAA23050.010.3026271
TCGCAGG116450.09.98389235
ATCGCCC184600.09.2440896
TCGCCCC169700.09.1967527
AGGGCGA96250.09.0501084
CGGTGGC246100.08.6493362
CGATCTG74350.08.47224
CCTCGCA141550.08.18828933
GACGGTT94350.08.122931
ACGGTTC93750.07.9650122
TGCGACA75000.07.7533413
ACGTCGT45300.07.7472144
CGTACCG36850.07.66983948
Produced by FastQC (version 0.11.5)