FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84366935
Sequences flagged as poor quality0
Sequence length42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATC3716980.44057307522194566TruSeq Adapter, Index 14 (97% over 42bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT2122350.25156182336124927No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC1882770.22316444232565757No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAA1718210.20365917050323093No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA1673780.1983928893469936No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC1629490.1931432023695065No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA1372470.16267866078102755No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATG1270320.1505708367857621TruSeq Adapter, Index 14 (97% over 41bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA1166350.13824728846674353No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACT1120710.13283758619416483No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT940820.11151525179858675No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT892790.10582226318877176No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA890500.1055508298363571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA566450.034.585571
ATCGGAA591550.033.6472172
CGTCTGA604950.032.05925816
ACGTCTG614400.031.62185515
ACACGTC618100.031.54927313
CACGTCT628300.031.01687414
CACACGT648300.030.09641812
GCACACG676950.028.98241611
CGGAAGA720200.028.2021754
GAGCACA713050.027.8460399
AGCACAC720850.027.4630110
AGAGCAC734050.027.051948
TCTGAAC721250.027.01179918
ACAGTTC722350.026.7214132
GTTCCAT710650.026.49269935
AAGAGCA769350.026.0399327
CAGTCAC744900.026.00187527
AGTTCCA752650.025.6169734
TCGGAAG804650.025.4559763
TCCAGTC764550.025.27473425
Produced by FastQC (version 0.11.5)