FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26090561
Sequences flagged as poor quality0
Sequence length42
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC3330881.276660934964181TruSeq Adapter, Index 1 (100% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATG278320.10667459392689946TruSeq Adapter, Index 1 (97% over 41bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA266090.1019870749425434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA407550.034.945261
ATCGGAA416450.034.618632
CGTCTGA423250.033.2566416
ACGTCTG425700.033.1707615
CACGTCT432100.032.76674714
CACGATC382250.032.2043536
TCACGAT423100.032.11965635
CATCACG433850.032.05386433
ACACGTC447350.031.65778513
AGAGCAC454950.031.4098
ATCACGA441050.031.3554134
CACACGT452200.031.32210512
TCTGAAC453600.031.04704318
GCACACG459250.030.94662511
GAGCACA464250.030.7953139
ACATCAC456700.030.50929532
AAGAGCA470500.030.5010597
AGCACAC469600.030.34865210
GTCTGAA467550.030.16725517
GTCACAT464900.029.9828429
Produced by FastQC (version 0.11.5)