FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43979710
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC1725330.39230135896757845TruSeq Adapter, Index 9 (100% over 42bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACA801210.18217719034527513No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA790270.17968967962726448No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA508200.1155532858220302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA241100.034.459721
ATCGGAA246050.033.786652
CGTCTGA263450.030.53223216
ACGTCTG270850.029.87744115
AGTCACG272000.029.40862728
CGATCAG271950.029.26855533
ACGATCA272950.029.24042732
TCACGAT275600.028.97259330
CACGTCT286250.028.41429114
ACACGTC286800.028.3786313
CACGATC282450.028.2698531
GCACACG295400.027.6377111
CACACGT300250.027.16136212
CGGAAGA323500.026.437984
AGAGCAC317500.025.980338
GATCAGA309200.025.46895834
GTCACGA315900.025.29915229
TCAGATC256300.025.07872436
GAGCACA330150.025.0557989
AGCACAC331000.024.92090610
Produced by FastQC (version 0.11.5)