FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29984395
Sequences flagged as poor quality0
Sequence length42
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA855300.2852483766972787No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG653590.2179767175559153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG585480.19526156855924556No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC375690.12529517437320312No Hit
GATCAATCGCCCCCGGGGGTGCCTCCGGGCTCCTCGGGGTGC331590.1105875239437047No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG331400.11052415764933726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG59400.021.8703423
GGGCGAT54500.021.3277571
GATCAAT66100.020.0892681
CAATCGC68150.018.9577734
GGCGATC63300.018.5896362
ATCAATC74650.018.1734922
TATAGGG31200.014.88388436
AATCGCC90750.014.6344655
CGGTGGC274050.013.6299542
CGATCAA18200.012.1602621
CGATCTG103600.011.4807554
AGGGCGA96550.011.1822114
TGGCGCG320800.010.7199675
GGTGGCG370750.010.186763
CGGAGGG106200.010.0470681
GCGATCT122100.09.9470193
GTGGCGC355650.09.7850354
TGTAGGG42850.09.5351236
TCGCCCC142300.09.5240037
ATCGCCC155950.09.3014226
Produced by FastQC (version 0.11.5)