FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64876402
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1332750.20542908652671582No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1204470.18565610343187652No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT1166890.17986355038616353No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA1064100.1640195767946564No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT1052690.16226084794283135No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1032380.15913027975873262No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG951260.14662650373243571No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC928940.14318611565419426No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT860860.13269231545855456No Hit
CGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCC838570.12925655155783763No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC814540.12555258536069866No Hit
CCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCG805670.12418537020594946No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT793370.12228945742089704No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG784840.12097464961142573No Hit
CCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACC784370.12090220416354161No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT746550.11507265769763249No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG726830.11203303167151595No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT679480.10473453814531823No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG671860.10355999705409065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC189550.047.2907372
GGGCGAT202800.044.3236051
GGCGCGT480200.037.464715
GCGATCT260050.034.68443
GGTGGCG537050.033.5148962
CGGTGGC554950.033.1741451
GCGCGTG548150.032.8102656
TGGCGCG602150.029.8147434
CGCGTGC657200.027.3587277
CGATCTG339900.027.0938424
GTGGCGC703400.025.6864553
GCGTGCC735950.024.4505778
GCGACAT516950.023.518217
TGCGACA552250.021.8429766
CGTGCCT837900.021.668549
CTGCGAC605650.020.0199815
GCTGCGA625350.019.3897234
CGACATC711850.017.2725478
GGCTGCG715700.017.062283
TAGTCCC887950.017.0229516-17
Produced by FastQC (version 0.11.5)