FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43130345
Sequences flagged as poor quality0
Sequence length42
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1724790.399901739714811No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA1074590.24914940977170483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN754000.1748189122994495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG633730.14693367280043781No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG628010.14560746036230407No Hit
CCGGGCTCCTCGGGGTGCGCGGCTGGGGGTTCCCTCGCAGGG569600.13206479104213054No Hit
GATCAATCGCCCCCGGGGGTGCCTCCGGGCTCCTCGGGGTGC517700.12003149986395889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG87500.022.2111323
GATCAAT98050.020.385051
ATCAATC100100.020.3115352
GGGCGAT54550.020.0188581
CAATCGC98800.019.746014
GGCGATC71550.015.21402552
AATCGCC133450.014.9574975
AGGGCGA156300.013.4720564
CGGAGGG175000.012.0279921
TGGCGCG601250.011.1367783
CGGTGGC443200.010.7136642
TCGCAGG201650.010.61190135
GTGGCGC655600.010.3082512
GTGACGT69000.010.2468131
GGTGGCG681650.010.240371
ACGTCGT66700.010.17237954
TGACGTC69500.09.9154482
ATCGCCC238900.09.4333516
GAGGGCG249650.09.0606363
CGATCAA27850.09.0437421
Produced by FastQC (version 0.11.5)