FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16335491
Sequences flagged as poor quality0
Sequence length42
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGGTTCCCGCCCCCACGGCGCGGGGCGCACGCCACACG212310.12996854517565465No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA210170.12865851415179377No Hit
CCACACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCG177910.10891010254910612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9950.018.8193681
ATCGGAA11650.015.9148432
GTCGCGT8300.015.18499851
ACCGCTA12300.015.0738152
TCGCGTC7950.014.7176112
CGTCTGA13600.013.49991616
CCTACAA68800.012.50600435
ACGTCTG15500.012.193397515
GACGCAC22950.011.607727
CCCTACG74750.011.48618224
ACGCACC23050.011.4791828
ACCGGGA24050.011.37619432
TACGTTC75600.011.35700227
CCTACGT75600.011.35696825
CTACGTT76100.011.21142626
TACCCTA76400.011.190927522
CCGCTAA19100.011.1208953
TCCTACA78850.010.91192434
ACCCTAC80700.010.70612223
CACCGCT18750.010.5629731
Produced by FastQC (version 0.11.5)