FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68439972
Sequences flagged as poor quality0
Sequence length42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATC2863020.4183257117638797TruSeq Adapter, Index 13 (97% over 42bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT1274440.18621281727000122No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAAC1136460.16605208429950846No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA1060470.15494892370791735No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAA1010440.14763886811642762No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC972170.1420471066235971No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA967860.14141735768097627No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA870740.1272268200226616No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACT725990.10607689903788974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA359000.034.0906681
ATCGGAA370650.033.559632
CGTCTGA387300.031.3463616
ACGTCTG396800.030.5914115
ACACGTC400950.030.54899413
CACGTCT416100.029.38457314
CACACGT424900.028.8738712
GCACACG442250.027.87986211
CGGAAGA470850.027.154744
GAGCACA481650.025.971699
AGAGCAC489850.025.5737328
AGCACAC490000.025.50563210
GTCAAAT473500.025.46095335
CAGTCAA477300.025.40531533
TCTGAAC488400.024.92747918
TCGGAAG517350.024.7546773
CAGTCAC508000.023.86652227
ACAGTCA518800.023.50839232
AAGAGCA544400.023.3351617
TCAAATC481050.023.00728436
Produced by FastQC (version 0.11.5)