FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68439972
Sequences flagged as poor quality0
Sequence length42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGA2802520.4094858484161858No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG1858900.2716102806120376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1115810.16303484168579144No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1102080.1610287041029181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG82700.017.5855393
CGGTGGC695500.016.0018442
GGGCGAT82150.015.1417651
CAATCGC97000.015.0296934
GCGGTGG837350.013.859791
GGCGATC92100.013.6806372
GATCAAT113600.013.6436571
TGGCGCG850400.012.6880775
GGTGGCG894250.012.4388263
ATCAATC127050.012.2832242
GTGGCGC909150.011.9653594
GGCGCGT940350.011.5090166
AATCGCC143150.010.6618945
GCGTGCC1108350.09.7129999
CGCGTGC1120050.09.6822118
CGTGCCT1153000.09.3650310
CGATCTG138350.09.275714
GCGCGTG1195850.09.0773617
CCTGTAG1217950.08.93669314
TGTAGTC1264450.08.59103416
Produced by FastQC (version 0.11.5)