FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76685012
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT4090780.5334523518102859No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2985390.3893055399143708No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2507280.3269582848862304No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2310580.30130790094940585No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2292510.29895150828169653No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2066380.26946334702275326No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1852270.24154263678018334No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1810830.23613871247747864No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1593950.20785678432181767No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1585500.20675487408152196No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1301090.16966679225400655No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1065500.13894501314024701No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC1038270.1353941236913414No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1023170.13342502965246977No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG873470.1139036139161066No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC850880.11095779707252311TruSeq Adapter, Index 5 (100% over 50bp)
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG818620.10675097762258941No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT785000.10236680930557852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA344750.032.690271
TCGCTAT354650.031.8250032
CGCTATG383150.029.0114233
CGTACGC82000.027.921945
CCGTACG86600.026.5489254
ACCGCGT209850.025.5972616
CGCGTTC214100.024.9781638
CCGCGTT224750.023.794617
GTCTGGA1365400.023.1020721
TGGAGTC1778300.022.9808942
GGAGTCT1791300.022.9679223
GACCGCG234350.022.9453075
GAGTCTT1842600.022.3566994
AGACCGC240900.022.22324
AGTCTTG1879300.022.1699855
GTACGCC106000.021.9100256
GTCTTGG1951950.021.5140276
CTTGGAA2010300.020.8139158
TTGGAAG2073250.020.1933989
GCTATGT567350.019.9606484
Produced by FastQC (version 0.11.5)