FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76685012
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1511100.1970528478237703No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA1116940.1456529732302839No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT1056150.13772573967909138No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1037060.1352363353610742No Hit
CCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCT996390.1299328218139941No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA959490.12512092975873826No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG933370.12171478828222652No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC900830.11747145583024751No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT813050.10602462968904536No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG808450.10542477322687253No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG802300.10462279121766324No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC800100.10433590334445016No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT788530.10282713393850679No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT777390.1013744380714187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC180950.043.5792472
GGCGCGT470700.037.8879975
GGGCGAT209550.037.5899051
GCGCGTG551250.032.37616
GGTGGCG557550.032.061262
GCGATCT253450.031.8207973
CGGTGGC593900.030.8459471
TGGCGCG604950.029.456914
CGCGTGC663550.026.8608937
CGATCTG324000.025.0519124
GTGGCGC737550.024.419823
GCGTGCC737600.024.2414918
GCGACAT454250.021.3803047
CGTGCCT861050.020.9537669
TGCGACA489500.019.743686
CTGCGAC535300.018.1173385
ACCTAGC190150.017.932656
GCTGCGA559000.017.3665244
CACCCGG268750.016.9882761
GACCTAG213550.016.70235
Produced by FastQC (version 0.11.5)