FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60982875
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG267050.017.7558175
CTATACT187850.015.5854344
TTATACT303350.015.4451894
ATAATAC269350.015.1441473
TAATACT286150.014.9612094
GTATATA328200.014.86694051
TATACAG259050.014.8029615
GTATTAT267050.014.7758581
GTATAAG158400.014.5834651
GTATTAA256100.014.5608491
GTATTAG154400.014.3200771
ATTATAC254050.014.1879043
CCTATAC119750.014.0883783
TCTATAC173150.013.9502733
TATATAC343550.013.55581953
GTATAGG118200.013.4809051
GTGTATA232650.013.3537171
TACACTG301100.013.344355
CTAATAC168000.013.3149563
GTATAGA178400.013.2128021
Produced by FastQC (version 0.11.5)