FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60982875
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG257600.017.0606525
GTCGCCG210250.015.6688194
CGCCGTA91900.015.59690194
GTATTAG156200.015.5877671
TATACAG259150.015.3443695
CTATACT180600.015.2522024
GTATATA326300.015.0680761
GTATAGG121500.014.9618551
GTATTAT272700.014.8175551
ATAATAC271650.014.7087633
GTATTAA265600.014.6994421
TTATACT303700.014.5800434
TCTATAC168500.014.3114763
GTATAGA175750.014.1217871
ATTATAC252000.014.1208963
GGTCGCC266900.014.0158294
GTATAAG160650.013.9540611
TGGTCGC285700.013.78439794
TAATACT283550.013.4942094
TCGCCGT160400.013.41884494
Produced by FastQC (version 0.11.5)