FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51344195
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG186400.016.5642875
CTATACT143400.014.6170374
GTATATA240800.014.553611
TATACAG187800.014.2637135
CCTATAC90000.013.995133
GTATAGA133950.013.8013251
TTATACT239950.013.788774
GTATTAG121950.013.1149931
TAGACTG173900.013.1067645
CTAGGAC149950.013.1013013
GTATAGG87350.012.8708111
GTATTAA207350.012.8405421
TCTATAC133950.012.7364543
ATTATAC196200.012.7197963
GTATTAT206900.012.6865831
TATATAC237650.012.6371073
CTAATAC126750.012.570033
ATAATAC209150.012.5614163
GTGTATA171150.012.56059551
TAATACT224300.012.5088864
Produced by FastQC (version 0.11.5)