FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51344195
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG187600.016.1598055
CTATACT138450.015.68405154
GTATTAG117800.014.997631
GTATATA240300.014.8216151
TCTATAC131250.014.4316163
GTATAGG87500.014.2841271
TTATACT239100.013.8585814
TATACAG186600.013.4253245
GTATTAT222750.013.4158911
CCTATAC90550.013.3918493
GTATAAG126400.013.2337531
CTAGACT139050.012.9798444
GTATAGA132250.012.9681281
CTAATAC127000.012.9530953
GTATTAA212700.012.8347881
TACACTG221050.012.7788915
ATAATAC205900.012.6234493
TAGACTG169050.012.6226335
GTACTAG69450.012.5164041
GTGTATA174700.012.5066251
Produced by FastQC (version 0.11.5)