FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68140766
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG280150.017.229315
GTATTAG165700.015.8971721
CTATACT194200.015.8813144
GTATATA340050.015.1608011
TATACAG276000.015.0021985
TCTATAC185350.014.914773
TTATACT324100.014.7962944
CCTATAC132100.014.6987043
ATAATAC285850.014.1117523
GTATTAT281900.014.0665271
TAATACT308500.014.0662524
CTAATAC173750.013.989353
ATTATAC268900.013.9697233
GTATAAG174300.013.9523551
GTATAGA184950.013.7847641
GTATAGG126650.013.5191871
GTATTAA281900.013.1654681
TACACTG327500.013.0592485
CTAGGAC197850.012.9981963
GTGTATA248300.012.8442091
Produced by FastQC (version 0.11.5)