FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61950258
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG266450.017.8154185
TACACTG327150.017.3400655
GTATTAG152350.016.0815641
CTACACT249400.015.9806214
CTATACT195450.015.9430754
TCTATAC171000.015.7216713
GTATAAG157650.015.6900651
TATACAG266450.015.6634575
GTATAGG133300.015.6281151
CCTACAC252250.015.14813
GTGTAGG274400.015.0639121
CTAGGAC213950.014.8941663
GTATAGA185000.014.7431151
TACACAG440100.014.5984755
TAGACAG311150.014.5461095
CCTATAC131800.014.2284073
GTGTTAG213900.013.91636851
TAGACTG229250.013.8793695
GTATATA367700.013.6971081
GTCCTAG208350.013.65511
Produced by FastQC (version 0.11.5)