FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62041406
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG265300.017.6501565
CTATACT196200.015.8710314
TACACTG318950.015.4911555
CCTATAC132650.015.4018413
GTATAGG130850.015.1514061
TCTATAC179300.014.8260953
TATACAG271550.014.8225085
GTATTAG165200.014.70262051
GTATAAG162250.014.5645641
GTATAGA181200.014.3377551
ATAATAC266150.013.8526813
GTATATA372900.013.7576571
CTACACT246550.013.7347554
TAATACT280200.013.6944494
TTATACT297000.013.6788724
TAGACTG230050.013.4540745
GTGTAGG272850.013.3441821
TACACAG433900.013.2381555
CCTACAC248500.013.0032743
CTTACAC231700.012.97312453
Produced by FastQC (version 0.11.5)