FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42849414
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG155250.016.5880265
CTATACT115150.014.9375264
TATACAG159400.014.917915
GTATATA195050.014.5188881
GTATAGG76000.014.2362981
TCTATAC106500.014.07723
GTATTAG98350.014.0622831
GTATAAG99950.013.9783691
ATTATAC166000.013.872773
CCTATAC74400.013.4549433
GTGTATA136250.013.39609051
GTGTAGG156550.013.3417491
CTACACT142750.013.2675414
TTATACT199950.013.1151824
GTATAAA220700.013.065951
TACACTG177700.012.9849375
CTAGGAC123750.012.8744793
TAGACTG135500.012.8670925
GTATACG22700.012.8483891
GTATTAT179550.012.81168651
Produced by FastQC (version 0.11.5)