FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42849414
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG152250.016.5466295
TATACAG153400.015.9629965
CTATACT118600.015.8517554
GTATAGG76350.015.4462721
GTATTAG100050.015.12161
CCTATAC78650.014.8202323
TACACTG181750.014.5074295
GTATAAG104550.014.111221
ATAATAC165800.013.9187113
GTATTAT178650.013.9127061
TTATACT200300.013.7270414
GTATTAA177650.013.5149561
GTATATA189850.013.3889221
TCTATAC108850.013.2991633
CTACACT146400.013.2911114
GTGTATA134950.013.1606661
TAATACT185650.013.1393674
GTGTAGG155900.012.7784661
GTATAGA108200.012.6364381
GTATAAT168850.012.5219051
Produced by FastQC (version 0.11.5)