FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92950584
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG445950.018.1475285
CTATACT300700.016.318014
GTATTAG250700.015.0394641
TTATACT487200.014.9432234
TCTATAC276800.014.8575443
TACACTG482500.014.2598265
TATACAG414000.014.15586955
GTATATA587950.014.1129111
GTATAAG264800.014.0078441
GTATTAT446600.013.8005461
TAATACT460650.013.7434774
ATAATAC442800.013.5228063
GTATAGG190500.013.4990951
TAGACAG463950.013.3408985
ATTATAC416050.013.2964583
CTACACT346550.012.9520234
CTAATAC270150.012.8745413
TATACCG50200.012.8258845
GTATAGA287950.012.8000381
GTATTAA408900.012.7044971
Produced by FastQC (version 0.11.5)