FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71716321
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG307550.019.0430745
TACACTG376550.015.5161545
CTATACT230650.015.4348394
TATACAG320700.015.0989265
GTATAGG149900.014.5737371
CTACACT281350.014.4896924
TTATACT348100.014.4366394
GTATTAG177250.014.2598721
CCTATAC157200.014.012213
GTATAGA210000.013.915241
TACACAG496800.013.736325
GTATATA432900.013.7176291
GTGTATA310100.013.6351621
GTATTAT319800.013.6329271
GTGTAGG313550.013.3652671
TAGACAG345850.013.3626825
TAATACT326800.013.3368194
CTAGGAC243450.013.330753
GTATAAG190050.013.1758591
CCTACAC280650.013.1703113
Produced by FastQC (version 0.11.5)