FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71716321
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG305400.016.8668385
CGCCGTA100800.016.81436594
GTATAGG154350.015.9844041
CTATACT222400.014.9326494
TATACAG313250.014.9081675
GTATAAG191800.014.8309891
TACACTG373400.014.7300545
GTATTAG186950.014.660611
TTATACT349850.014.61664
GTATATA425050.014.516741
CCTATAC155050.014.3605183
GTACTAG110450.014.0944991
GTGTATA301350.013.8539461
TCTATAC196800.013.8069343
GTATAGA216400.013.711811
CTACACT287650.013.4477134
ATAATAC305500.013.4186373
CTAATAC192900.013.3035183
GTGTAGG307100.013.2719681
TAATACT328550.013.26694
Produced by FastQC (version 0.11.5)