FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69323748
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG273150.017.0343635
CTATACT187700.015.1290354
GTATAGG129950.014.9134331
GTATATA328750.014.8521151
TTATACT319400.014.660984
GTATTAG161150.014.653121
GTATTAT273150.014.3277771
TATACAG256600.014.2316855
GTATTAA265100.013.9821251
TCTATAC179700.013.8664733
ATAATAC272650.013.8640083
GTATAGA184700.013.7270931
GTATAAG168350.013.1882011
TATACTA170050.013.183595
CCTATAC132150.013.0923883
CTAATAC169400.012.9333413
ATTATAC262950.012.9271673
TAATACT293050.012.7705574
GTATAAT263450.012.7305751
TAATACA329900.012.7264624
Produced by FastQC (version 0.11.5)