FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66549852
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG280800.018.3444255
TACACTG348150.017.3876195
TATACAG287650.016.2900125
GTATAGG141800.016.0515251
CTATACT206850.015.8369124
CTACACT264100.015.4648114
GTATAAG173250.015.3906521
TCTATAC182750.015.2251363
GTATTAG166800.015.1399841
CCTATAC145450.014.864183
TAGACTG246750.014.5711875
TACACAG472150.014.5531845
CTAATAC178200.014.4270143
GTATAGA196800.014.4091841
CGTACAC93650.014.353413
GTATATA384550.014.2591271
GTGTAGG287450.014.2005311
GTCCTAG216550.014.0722661
TAGACAG326950.013.7712965
CTTACAC252000.013.689653
Produced by FastQC (version 0.11.5)