FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66549852
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG138450.017.7837071
TATACTG284950.017.6749345
CTATACT208300.016.6460254
GTATTAG164050.016.0969661
TATACAG286300.015.6716155
TCTATAC184200.015.4830283
GTGTAGG294150.015.4309341
TACACTG345900.015.4297715
CCTATAC147600.015.406923
CGCCGTA92150.015.35027694
GTATAAG175300.015.2247571
CTACACT267600.014.941254
GTATAGA195900.014.9190051
GTCCTAG222350.014.4333621
GTGTACG123650.014.3642051
TAGACTG238150.014.2435535
CTAGGAC221100.014.15283
CTAATAC177050.014.117983
GTATATA386150.014.0786591
CTAGTAC99800.014.07659153
Produced by FastQC (version 0.11.5)