FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78876939
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG313050.017.5681235
CTATACT226050.015.1878064
GTATATA394200.014.7541431
TACACTG370450.014.5290495
GTATTAT333100.014.5127841
GTATTAG187750.014.437961
TATACAG314250.014.3326165
TTATACT374700.014.13863854
GTATAAG195550.013.9821871
GTATAGA213600.013.39448451
CCTATAC151200.013.35668853
GTGTATA285550.013.260591
CTACACT271050.013.1168224
ATAATAC326200.013.1020613
GTATTAA321350.013.0551881
TCTATAC203700.013.04991153
ATTATAC313800.012.9612593
GTATAGG156400.012.7662141
TAGACTG264450.012.7337925
TAGACAG361100.012.6551575
Produced by FastQC (version 0.11.5)