FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78876939
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG311400.016.664755
CGCCGTA103450.015.38464194
GTATAAG195950.015.3128381
CTATACT223900.015.0658274
GTATTAG190200.014.8331881
GTATATA388100.014.7941751
GTATAGG147050.014.3091441
TTATACT368200.014.2867074
GTATTAT331250.014.14285851
CCTATAC156950.014.0077243
TATACAG317650.013.9011825
TCTATAC202850.013.7453393
ATAATAC335050.013.54591753
TACACTG369650.013.5409585
GTGTATA289950.013.3424181
GTATAAT316900.013.249871
GTATAGA213150.013.1696871
TAATACT357650.013.1647734
ATTATAC300550.013.1229823
CTAATAC199550.012.8614563
Produced by FastQC (version 0.11.5)