FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63923293
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG254450.017.231455
CTATACT185150.015.8381144
GTATATA315300.015.3852461
GTATTAT257600.015.2993681
GTATTAG156050.015.0445311
TATACAG254650.014.9849385
TTATACT303750.014.9452584
GTATAAG160250.014.7973181
ATTATAC258200.014.5240993
TCTATAC177250.014.5025263
ATAATAC268750.014.4435583
TACACTG296200.013.8982985
GTATAGA175550.013.8836221
TAGACAG317250.013.6130825
GTATTAA259950.013.5832931
TAATACT288100.013.5224024
TATACTA165400.013.2969775
GTATAGG122050.013.2099571
GTATAAT262200.013.1970381
TAGACTG230800.012.9701865
Produced by FastQC (version 0.11.5)