FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63923293
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG250700.016.6121865
GTATTAG159800.016.4451581
TATACAG253250.015.9252135
GTATTAT270250.015.858291
CCTATAC124150.015.7515353
GTATATA320650.015.656931
CTATACT190150.015.5744114
GTATAGG123200.015.4054711
GTATAAG161050.014.7676471
TCTATAC171200.014.6626933
TTATACT309250.014.5924754
ATTATAC254150.014.3716673
TACACTG302150.014.2190625
GTATTAA266150.014.1030311
CTAATAC167700.013.9315563
TAATACT289150.013.62348654
TGGTCGC233100.013.60862994
GTGTATA229650.013.5349961
CGCCGTA71950.013.455173594
GTATTAC171650.013.2795141
Produced by FastQC (version 0.11.5)