FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76630807
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA140950.017.940394
GTCGCCG308900.017.5438194
TATACTG345200.016.7054965
TCGCCGT226950.015.92607294
GTATTAG194500.015.3929611
GTATATA431850.015.1825111
CTATACT236050.015.1519694
GGTCGCC397750.014.98378794
TGGTCGC436150.014.69910994
GTATAGG150850.014.5815231
GCCGTAT136500.013.979959594
TATACAG319350.013.9664145
GTATAGA222550.013.9174641
TTATACT390150.013.8292534
GTATTAA329150.013.7652941
ATAATAC337100.013.5800253
GTATTAT339450.013.4445331
CCTATAC151900.013.3977083
TAATACT371500.013.3596034
TCTATAC222650.013.1512093
Produced by FastQC (version 0.11.5)