FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68140771
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG297950.018.3296175
TACACTG362450.016.6497425
GTATAGG150200.015.9362051
CTATACT211500.015.8220864
TATACAG300550.015.6220995
GTATTAG172500.015.6207751
CCTATAC147750.015.4916453
GTATAAG182650.015.3191391
CTACACT280850.015.1784344
TACACAG485400.014.9886265
CTAGGAC238100.014.7849043
TAGACAG344950.014.7421825
GTGTAGG295650.014.5698351
GTCCTAG222650.014.3833961
GTATATA424600.014.3536411
GTGTTAG239150.014.157911
TCTATAC191400.014.1441423
CCTACAC276550.014.07193
CTTACAC255900.014.0687183
GTGTACG132950.014.006961
Produced by FastQC (version 0.11.5)