FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68140771
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG296800.018.8532815
CGCCGTA124150.018.4723194
GTATAGG145700.017.1250461
GTATTAG175950.017.011671
GTCGCCG292550.016.48144794
CTATACT209400.016.1998884
TACACTG357150.015.9043055
GTGTAGG298650.015.8439591
TATACAG298500.015.3776265
GTATAAG177000.015.2117131
CCTATAC150500.015.1727633
CTACACT275800.015.0253924
CTAGGAC231350.015.0085883
CCTACAC272800.014.9327443
GTATAGA209700.014.856341
TCGCCGT207650.014.80109694
GTGTTAG234600.014.6615491
GTCCTAG226350.014.5523891
GGTCGCC366750.014.5308594
TGGTCGC391750.014.49125594
Produced by FastQC (version 0.11.5)