FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61259645
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG261200.018.0272835
CTATACT181600.015.5522914
TATACAG258150.015.5278965
GTATATA323000.015.2514331
GTATTAG155150.015.2223911
TTATACT299900.015.1055414
ATTATAC251900.014.6632453
GTATTAT270550.014.5316671
GTATAAG155700.014.2905941
GTATTAA262950.014.1090731
TCTATAC171550.014.0801773
TAATACT284300.014.0665774
ATAATAC270650.014.0641253
GTATAGA176300.013.9310011
CCTATAC117300.013.621243
GTATAGG116850.013.5955921
TACACTG291800.013.5922985
TAGACAG302650.013.1671235
TATATAC326650.013.019723
TACACAG383250.012.9361795
Produced by FastQC (version 0.11.5)