FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61259645
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG258750.017.4577985
CTATACT183650.016.1769524
GTATATA319200.015.4770721
TTATACT301700.015.4407694
GTCGCCG192700.015.0229894
CGCCGTA80400.015.022222594
GTATTAA263600.014.9360951
TATACAG249950.014.8942455
TGGTCGC252950.014.78888794
GTATTAT274100.014.707571
GTATTAG156250.014.5580531
GTATAGG118200.014.2640131
TCTATAC166650.014.1605573
GTATAAG152550.014.1393511
ATTATAC251300.014.0297753
CCTATAC120650.014.02675153
TACACTG290300.013.73078255
GGTCGCC238800.013.61848794
GTATAGA176350.013.4328671
ATAATAC269100.013.398733
Produced by FastQC (version 0.11.5)