FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72669922
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG314100.016.0853185
GTATTAG186250.014.4803521
GTATATA421700.014.42878151
TATACAG300600.014.2591955
CTATACT227350.014.2227194
GTATAGA213650.014.1627141
TCTATAC198950.014.1268843
TACACTG367750.013.98155
GTATAAG189950.013.7530491
GTATAGG151100.013.4643981
GTATACG38450.013.4418751
CTAGGAC232100.013.2229153
ATAATAC314550.013.1785633
CCTATAC145950.013.1384843
TTATACT352300.013.1271864
GTATTAT328800.013.0896241
TAGACTG255900.012.8930595
CTACACT285100.012.8419044
GTGTAGG307050.012.7161451
GTATTAA307650.012.7066171
Produced by FastQC (version 0.11.5)